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Gene distribution and SSR markers linked with net type net blotch resistance in barley

Gupta, S., Wielinga, C., Li, C., Cakir, M., Platz, G., Loughman, R., Lance, R. and Appels, R. (2004) Gene distribution and SSR markers linked with net type net blotch resistance in barley. In: 9th International Barley Genetics Symposium, 20 - 26 June, Brno, Czech Republic pp. 668-673.

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Net type net blotch caused by Pyrenophora teres f. teres is a major disease in Western Australia which reduces significant barley production around the world. Studies were focussed on four resistant lines to identify microsatellites linked with the resistance. The four lines, WA 4794 (103 IBON 91) (Pedigree: Arupo S × 2/3/PI 2325/Maf 102//Cossack), Pompadour (Pedigree: FDO192/Patty), CI 9214 (Pedigree: Collected from South Korea) and WPG 8412-9-2-1 (Pedigree: Bowman//Ellice/TR451) were crossed with Stirling (Pedigree: Dampier//Prior/Ymer/3/Piroline), a susceptible but well adapted cultivar in Western Australia. Doubled haploid (DH) populations were generated through another culture. In case of WA4794, two genes were mapped on 4H and 6H using the microsatellite markers GMS089, Bmag0384 for 4H, and Ebmac0874 for 6H. In Pompadour population, two NNB resistance genes were mapped on 3H and 6H using the microsatellites Bmac0209 and Bmag0173 respectively. In CI 9214, Bmac0218 was linked with the resistance for 2H, Ebmac0871 with 3H, suite of microsatellites Ebmac0635, Ebmac0701 and Ebmac0788 with 4H, and similarly Bmag0173, Bmgtttttt1, Ebmac0874 and HVM74 with 6H. In case of WPG 8412/Stirling, single gene was mapped on 6H using the microsatellite Bmag0173. The R2 value ranged up to 0.80 for the linked microsatellites and some are closely mapped to the resistance genes.

Publication Type: Conference Paper
Murdoch Affiliation: Western Australian State Agricultural Biotechnology Centre
Notes: In Spunar, J. and Janikova J. (2004) Proceedings (Part II) of the 9th International Barley Genetics Symposium, Brno Czech Republic, pp 668-673
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