Strongyloides ratti: Fumarate reductase and succinate dehydrogenase activities of infective larvae
Armson, A., Grubb, W.B. and Mendis, A.H.W. (1993) Strongyloides ratti: Fumarate reductase and succinate dehydrogenase activities of infective larvae. International Journal for Parasitology, 23 (6). pp. 809-811.
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Submitochondrial particles prepared from axenised infective (L3) larvae of S. ratti (homogonic-strain) were assayed spectrophotometrically for fumarate reductase (FR) and succinate dehydrogenase (SDH) and their kinetic properties characterised. The S. ratti FR (pH 8.2; 37°C) exhibited a maximum specific activity of 3.45 nmol (min)−1 (mg protein)−1 at a sodium fumarate concentration of 0.3 mm. Interestingly, the FR activity declined at fumarate concentrations greater than 0.3 mm. The mechanism of this unusual inhibitory effect requires further study. The S. ratti SDH (pH 8.2; 37°C) showed a Vmax of 17.4 nmol (min)−1 (mg protein)−1; the Kmsucc was 0.5 mm. Although the SDH:FR ratio cannot predicate vectorial electron flow as would occur in vivo, an in vitro ratio of 5.04:1 was observed for SMPs derived from S. ratti L3 larvae.
|Publication Type:||Journal Article|
|Copyright:||1993 Australian Society for Parasitology|
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