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HPIC analysis of phosphite and phosphate extracted from plant samples

Loane, C., Roos, G., Hardy, G., Dell, B., Colquhoun, I. and McComb, J. (1997) HPIC analysis of phosphite and phosphate extracted from plant samples. In: 11th Biennial Conference of the Australasian Plant Pathology Society, 29 September - 2 October, Perth, Western Australia.

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Diseases caused by members of the genus Phytophthora have, for a long time, been recognised globally as among the most devastating plant diseases. In Australia, diseases caused by Phytophthora spp. cause widespread losses in pastoral, agricultural, horticultural, ornamental and forestry industries, and cause loss of biodiversity in national parks and nature reserves throughout the country.

The chemical control of Phytophthora a diseases in Australia primarily involves systemic fungicides based on phosphonic acid: the active constituent being the phosphonate (phosphite) anion. The use of phosphite fungicides has had a significant impact on disease control within Australia. Although the mechanism of action of phosphite remains to be clarified, its effectiveness to inhibit growth and sporulation of pathogenic agents motivates the search for a method of determining this anion in biological systems.

In the past, analysis has been achieved by Paper Chromatography (PC), Gas Chromatography (GC) and High Performance Ion Chromatography (BPIC). Most procedures are tedious and costly, HPIC columns degraded rapidly and in many cases are no longer available. The advent of new HPIC resin columns was heralded as the answer to the problematic analysis of biological phosphite extracts, however the current investigation proved this not to be the case.

The current investigation, which. is based on previous research, offers a robust, rapid and long term analytical method for the analysis of biological samples which contain both phosphite and phosphate.

Publication Type: Conference Item
Murdoch Affiliation: School of Biological and Environmental Sciences
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