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Dissecting the infection machinery of Stagonospora nodorum using DNA microarrays

Ipcho, S.V.S., Hane, J.K., Solomon, P.S. and Oliver, R.P. (2008) Dissecting the infection machinery of Stagonospora nodorum using DNA microarrays. In: 9th International Congress of Plant Pathology ICPP 2008, 24 - 29, Torino, Italy.

Abstract

The fungus Stagonospora (Septoria) nodorum [teleomorph Phaeosphaeria (Leptosphaeria) nodorum] is an important pathogen of cereals, causing both leaf and glume blotch. The genome sequence of this fungus is composed of 16116 genes, of which 10762 genes were predicted with a high level of confidence. RNA was extracted from lesions corresponding to early proliferation, vegetative state and sporulation on wheat leaves, and from mycelia of the fungus grown on minimal media reflecting vegetative state and sporulation. Based on the annotation of the genome sequence, a whole genome microarray was designed. Microarray experiments were undertaken using Cy3-labelled cDNA derived from the mentioned samples (Nimblegen Inc.). This robust methodological approach provided the expression profile of the pathogen during the key stages of plant infection and in vitro growth. These expression profiles were analysed and a subset of differentially expressed genes identified. Potential pathogenicity genes occurring as single copy in the genome and with interesting expression profiles were chosen for further study using a reverse genetics approach.

Publication Type: Conference Item
Murdoch Affiliation: Australian Centre for Necrotrophic Fungal Pathogens
URI: http://researchrepository.murdoch.edu.au/id/eprint/12161
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