Effects of temperature and duration of sample storage on the haematological characteristics of western grey kangaroos (Macropus fuliginosus)

Hulme-Moir, K.L., Clark, P. and Spencer, P.B.S. (2006) Effects of temperature and duration of sample storage on the haematological characteristics of western grey kangaroos (Macropus fuliginosus). Australian Veterinary Journal, 84 (4). pp. 143-147.

Abstract

Objective: To investigate the effects of storage duration and temperature on haematological analyses performed on blood from the western grey kangaroo (Macropus fuliginosis). Method: Blood samples from five western grey kangaroos were stored at 4°C, 24°C and 36°C. Each sample was analysed haematologically over a 5-day period. Results: The blood samples maintained optimal stability at 4°C. At this temperature the haematological values remained essentially unchanged for the duration of the study, while samples stored at 36°C and 24°C showed significant changes in some haematological measures by 12 h and 48 h, respectively. Disturbances in leukocyte morphology were evident, to varying degrees, in all samples. Conclusions: Blood samples from macropodids should be tested within 48 h of collection if stored at a room temperature of about 24°C. Where testing is to be delayed for more than 48 h, samples should be refrigerated as soon as possible. Exposure of samples to heat in excess of 24°C should be avoided at all times.