Catalog Home Page

Adipocyte fatty acid-binding protein and mitochondrial enzyme activities in muscles as relevant indicators of marbling in cattle

Jurie, C., Cassar-Malek, I., Bonnet, M., Leroux, C., Bauchart, D., Boulesteix, P., Pethick, D.W. and Hocquette, J.F. (2007) Adipocyte fatty acid-binding protein and mitochondrial enzyme activities in muscles as relevant indicators of marbling in cattle. Journal of Animal Science, 85 (10). pp. 2660-2669.

[img]
Preview
PDF - Published Version
Download (130kB) | Preview
    Link to Published Version: http://dx.doi.org/10.2527/jas.2006-837
    *Open access, no subscription required

    Abstract

    Marbling is an important criterion for beef quality grading in many countries. The purpose of the current study was to utilize the natural genetic variation to identify major metabolic indicators of marbling in cattle differing in genotypes. Rectus abdominis (RA, oxidative), semitendinosus (glycolytic), and longissimus thoracis (LT, oxido-glycolytic) muscles were taken from steers of different genotypes that expressed high [Angus, n = 16; and crossbred (Angus x Japanese Black), n = 10] or low (Limousin, n = 12) levels of marbling in their meat. Muscles from Angus and crossbred steers were characterized, as expected, by a greater triacylglycerol (TAG) content (P < 0.001) and also by greater protein contents of fatty acid-binding protein specific for heart and muscles (H-FABP; P < 0.001 for RA and P < 0.05 for LT muscle) or for adipocytes (A-FABP; P < 0.001 for RA and LT muscles). Moreover, oxidative enzyme activities (β-hydroxyacyl-CoA dehydrogenase, citrate synthase, isocitrate dehydrogenase, cytochrome-c oxidase) were greater (P < 0.01 to 0.001) in the 3 muscles studied, whereas glycolytic enzyme activities (phosphofructokinase and lactate dehydrogenase) were lower (P < 0.001) in RA muscle in Angus and crossbred steers compared with Limousin steers. Significant correlations were observed between TAG content and H- and A-FABP protein contents, and oxidative (r ≥ +0.55, P < 0.001) or glycolytic enzyme activities (r ≥ -0.47, P < 0.001), when the 3 genotypes and muscles studied were considered as a whole. In addition, A-FABP protein content and some oxidative enzyme activities were significantly correlated with TAG content independently of the genotype and muscle effects. In conclusion, A-FABP protein content, as well as oxidative enzyme activities, may be used as indicators of the ability of steers from extreme genotypes to deposit intramuscular fat.

    Publication Type: Journal Article
    Murdoch Affiliation: School of Veterinary and Biomedical Sciences
    Publisher: American Society of Animal Science
    Copyright: ©2007 American Society of Animal Science. All rights reserved.
    URI: http://researchrepository.murdoch.edu.au/id/eprint/11004
    Item Control Page

    Downloads

    Downloads per month over past year