Evaluation of the dual IFNγ/IL-2 fluorospot-assay with flow cytometry for detection of HLA-restricted HIV-specific T-cell responses in HIV controllers
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Keane, N., Almeida, C., Roberts, S., Ahmad, I., Mallal, S. and John, M. (2012) Evaluation of the dual IFNγ/IL-2 fluorospot-assay with flow cytometry for detection of HLA-restricted HIV-specific T-cell responses in HIV controllers. Retrovirology, 9 (Suppl 2). P243.
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Abstract
The IFNγ ELISpot assay is used widely for high through-put screening of HIV-specific responses in studies of HIV infection and vaccine studies. However, dual production of IFNγ/IL-2 and increased proliferative capacity may be associated with better natural control of HIV infection. We evaluated a novel fluorospot assay enabling the identification of dual IFNγ/IL-2 producing antigen-specific cells and compared it with intracellular cytokine staining by standard flow cytometry in individuals with natural control of HIV-infection.
| Publication Type: | Journal Article |
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| Murdoch Affiliation: | Institute for Immunology and Infectious Diseases |
| Publisher: | BioMed Central |
| Copyright: | © 2012 Keane et al |
| Notes: | Poster Presentation at AIDS Vaccine 2012, Boston, 9-12 September, 2012. |
| URI: | http://researchrepository.murdoch.edu.au/id/eprint/10928 |
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