The response of intact Strongyloides ratti infective (L3) larvae to substrates and inhibitors of respiratory electron transport
Mendis, A.H.W., Armson, A., Thompson, R.C.A. and Grubb, W.B. (1991) The response of intact Strongyloides ratti infective (L3) larvae to substrates and inhibitors of respiratory electron transport. International Journal for Parasitology, 21 (8). pp. 965-968.
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Live, intact third-stage larvae (L3s) of Strongyloides ratti in the absence of exogenous substrates consumed oxygen at a rate (E-QO2) of 181.8 ± 12.4 ng atoms min-1 mg dry weight-1 at 35°C. Respiratory electron transport (RET) Complex I inhibitor rotenone (2 μM) produced 33 ± 6.5% inhibition of the E-QO2. Unusually the rotenone-induced inhibition was not relieved by 5 mM-succinate. The E-QO2 of intact L3s was refractory to RET Complex III inhibitor antimycin A at 2 μM; 4 μM-antimycin inhibited ≤10% of the E-QO2. The electron donor couple ascorbate/TMPD augmented the E-QO2 in the presence of rotenone (2 μM) and antimycin A (4 μM) by 110%. Azide (1 mM) stimulated the antimycin A refractory QO2 by 36.6 ± 7.2% which was only partially inhibited by 1.0 mM-KCN (IC50 = 0.8 mM). The data suggest the presence of classical (CPW) and alternate (APW) electron transport pathways in S. ratti L3s.
|Publication Type:||Journal Article|
|Murdoch Affiliation:||School of Veterinary Studies|
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